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Preparation 6 min read

Reconstituting a lyophilised vial

Lyophilised peptides ship dry because dry is stable. Bringing one into solution takes two minutes and one decision: how concentrated you want it.

HP Written by the Hello Peptides lab team · Updated August 2026

What you need

  • The lyophilised vial, at room temperature
  • Bacteriostatic water (0.9% benzyl alcohol). Sterile water works too, but the solution then keeps for days rather than weeks
  • A sterile syringe and needle
  • Alcohol prep wipes

Method

  1. 01
    Wipe both stoppersSwab the peptide vial and the water vial. Let the alcohol dry; it needs contact time to work.
  2. 02
    Draw the waterPull the volume you have chosen into the syringe. 1–3ml is typical for a 10mg vial.
  3. 03
    Add it slowlyAngle the needle so the water runs down the inside of the glass rather than jetting onto the powder.
  4. 04
    Swirl, never shakeRoll the vial gently between your fingers until the powder disappears. Shaking foams the solution and denatures the peptide.
  5. 05
    Label itWrite the concentration and the date. Store at 2–8°C and use within 28 days.
Pre-mixed vials skip all of thishello lean and our single-compound vials arrive in solution. There is nothing to add; store them cold and they are ready.

Working out the concentration

Concentration is simply the mass in the vial divided by the water you add. The calculator below does the arithmetic for the volumes you are likely to use.

Research use onlyProducts are supplied for laboratory research and are not for human or animal consumption, diagnosis or treatment. Questions about a batch? Contact the lab with the batch number.
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Storage Storing peptides Lyophilised or pre-mixed, fridge or freezer, and how long each keeps. 5 min read Handling Aseptic handling in the lab Keeping vials sterile from first puncture to disposal. 4 min read Quality Reading a batch certificate What the HPLC trace, mass spec and purity figure actually tell you. 5 min read